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NDT.net Issue - 2016-02 - Articles
NDT.net Issue: 2016-02
Publication: 6th Conference on Industrial Computed Tomography (iCT) 2016, 9-12 February 2016, Wels, Austria (iCT 2016)
Session: Short talks (5 mins)
ARTICLE

Industrial Tomography System for Answering Biological Issues: Development of the Mouse Embrya Face.

Dominika Kalasová13, Tomáš Zikmund19, Marketa Tesařová12, Josef Jaroš2, Markéta Kaucká32, Igor Adameyko32, Jozef Kaiser19
1Central European Institute of Technology; Brno University of Technology (BUT)227, Brno, Czech Republic
2International Clinical Research Center - Center of Biomolecular and Cellular Engineering; St. Anne's University Hospital Brno, Brno, Cyprus
3Department of Physiology and Pharmacology; Karolinska Institutet3, Stockholm, Sweden

Abstract: Understanding of some biological processes like a face development requires among others the high-resolution 3D imaging of increasingly complex cartilage in verterate embryos. During the face development, cartilages are produced in a variety of shapes and sizes making a convenient model system. X-ray computed microtomography (microCT) imaging is limited by the low inherent contrast of non-mineralized tissues. Although X-ray contrast enhancement agents are used routinely in clinical radiography, only a few techniques have appeared for imaging soft tissues in preserved animal specimens. Here we present a feasibility study of three different tomographic systems for high resolution and high contrast imaging of embryonic tissues. The industrial system equipped with flat-panel is compared with microCT system using synchrotron radiation (SYRMEP beamline of Elettra, Italy) and laboratory-based micro/nano CT system (Rigaku, nano3DX) based on Motarget and CCD camera. The mouse embryos were stained by phosphotungstic acid which produced overall contrast and differential tissue contrast. Furthermore, we demonstrate the utilisation of the standard industrial tools (wall thickness analysis and 3D printing) which can help to understand the differences or similarities among of different mouse embryonic development stages.

Keywords: Wall Thickness Analysis (2), industrial tomography (2), lab-based nanotomography, synchrotron-based microtomography, mouse embryo, 3D printing (10),
*Keywords are freely formed keywords from the authors and thus you may Search also for similar terms.

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